Abstract：Homologous chromosome pairing, synapsis and genetic recombination are achieved in meiosis. Meiotic recombination is the main source of genetic variation in sexually reproducing organisms because the recombination speeds up adaptation, and lay the foundation for traditional plant breeding. The recombination is initiated by the programmed DNA doublestranded breaks (DSBs) that are processed to form chromosome crossovers (COs). DSBs and COs predominantly occur at discrete genomic loci called recombination hotspots. The knowledge about the distribution of recombination hotspots in plant genome as well as the features associated with these areas is invaluable to breeding, yet still largely unknown. Here, methodologies to profile hotspots at different steps of the meiotic recombination pathway, their contributions to understanding of hotspots and their limitations were reviewed. Novel and highefficiency approaches will not only facilitate the mapping of plant recombination hotspots, but also provide insights about the formation and the control of these hotspots.